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 Supplementary Notes

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 SupplementaryNotes_2

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 SupplementaryNotes_7

 SupplementaryNotes_8

 SupplementaryNotes_9    

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Note:

1. The two searches pertaining to the RNA-isolation methods (Supplementary notes 9, Tables 1 & 2) were performed in the month of September 2007, whereas, the search for obtaining the most widely used primer designing tools (Supplementary notes 9, Table 3) was performed in Sep/Oct-2009.

2. Though PubMed Central gave extremely large number of hits, the '% relevant hits' obtained were low. This was due to the limitation in the phrase search option of the tool, wherein, a phrase such as “primer designed using” was appended as “(primer[All Fields] AND designed[All Fields])”.

3. If one has to look for experimental protocols from articles, PubMed and other citation scanners pose a challenge. On the contrary, the full text scanners can effectively perform such a task with considerable ease. This is because, such specifics are often hidden in the method section, which is not accessed by the citation scanners. Also, many of the protocols are not the actual subject matter of the study, but are just a component of a bigger objective. Hence, while employing the citation/summary scanners to retrieve such articles, it is necessary to focus on the bigger objective of the study. This complicates the query designing process.
  Example: To determine the primer designing software used in recent years, from the existing literature, one can use a simple query such as “primers designed using” in the full-text scanners such as Google Scholar. However, the use of such a query in PubMed would result in a large number of irrelevant citations. This is primarily because, a) the information sought by the user is unlikely to be mentioned in the fields of citations, and PubMed cannot consider phrases consisting of stop words. Thus, a more extensive query directed at a larger aspect (such as amplification of a gene, cloning, etc.), has to be used.

4. Brij.in was not considered in the study, since it truncates the results without any indication of the total number of hits. Furthermore, it was found that the citations retrieved by the tool were most often covered by Google Scholar, though the initial trend seemed different. PubMed Central was considered for one of the initial searches regarding primer designing program/software, but was found to be ineffective.

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